Bicine is a commonly used buffer in laboratories, mainly used to maintain the pH stability of solutions. It plays an important role in protein research, enzyme experiments, and electrophoresis analysis. However, many people may not know that the stability of Bicine buffer is affected by temperature. If the storage method is not careful, it may lead to inaccurate experimental data. Today, let's talk about how to properly store Bicine buffer to ensure its optimal performance.
Basic characteristics of Bicine buffer solution
Bicine, also known as N, N-di (2-hydroxyethyl) glycine, is a mild buffering agent suitable for use in the pH range of 7.6-9.0. Its advantages are low toxicity and strong buffering capacity, so it is widely used in biological experiments. However, like many chemical reagents, Bicine is not completely stable, especially at higher temperatures where its effectiveness may be compromised.
The Effect of Temperature on Bicine
High temperature will reduce the stability of Bicine
If Bicine is placed in a high-temperature environment (such as room temperature in summer or near heating equipment), its molecular structure may change over time, leading to a decrease in buffering capacity. Experiments have shown that after being stored at 50 ° C for a few days, the pH value of Bicine solution may undergo significant changes, affecting the experimental results.
Low temperature storage can extend the lifespan of Bicine
On the contrary, if Bicine is stored in a refrigerator (4 ° C), its stability will be greatly improved. Bicine can remain unchanged for several months or even longer in low-temperature environments. If long-term storage is required (more than six months), freezing (-20 ° C) storage can also be chosen, but attention should be paid to avoiding repeated freeze-thaw cycles, otherwise it may affect the effectiveness of use.
How to store Bicine buffer solution correctly?
To ensure the optimal performance of Bicine, it is recommended to take the following measures:
1. Store in refrigerator: The prepared Bicine buffer is best kept in a refrigerator at 4 ° C to avoid high temperature environments.
2. Avoid light storage: Ultraviolet rays in sunlight may affect the stability of Bicine. It is recommended to use brown bottles or aluminum foil wrapped containers.
3. Prepare with purified water: Ordinary tap water may contain metal ions, which may accelerate the decomposition of Bicine, so it is best to use high-purity distilled water or deionized water.
4. Ready to use: If the experiment requires a high pH value, it is recommended to prepare it fresh before each use to avoid long-term storage.
5. Regularly check the pH value: If the buffer solution has been stored for a long time, it is best to measure the pH value again before use to ensure that it still meets the experimental requirements.
summarize
Bicine is a very practical buffer, but its stability is affected by temperature. High temperature may cause it to fail, while low-temperature storage can greatly extend its service life. Therefore, when using Bicine in the laboratory, it is important to pay attention to storage conditions to ensure that it always remains in its optimal state. This way, our experimental data will be more accurate and reliable.
Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of bicine buffering agents. The company was established in 2005 and has been committed to the research and development of biological buffering agents for more than ten years. Not only has it established its own research and development team, but it has also achieved important results in this field. Currently, the company produces and sells more than 30 types of biological buffering agents, with a complete range of products. Welcome customers who are interested in purchasing to inquire and learn more!
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